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A comparison of blood vessels imaged with short-wave fluorescence imaging right and near-infrared fluorescence imaging left. Both images rely on a fluorescent dye called ICG, but the vessels can be seen more clearly with short-wave fluorescence imaging. It is also commonly used to image blood vessels during reconstructive surgery, allowing surgeons to make sure the vessels are properly connected.

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Cutting through the fog The dye that the researchers used in this study, known as indocyanine green ICGfluoresces most strongly around nanometers, which falls within the near-infrared range. When injected into the body, it travels through the bloodstream, making it ideal for angiography the visualization of blood flowing through vessels. Some robot-assisted surgical systems have incorporated NIR fluorescence imaging to help visualize blood vessels and other anatomical features. Light with shorter wavelengths tends to scatter off of imperfections in objects that it strikes, but as wavelengths become longer, scattering is greatly reduced.


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